Aelton Silva Araujo

Undergraduate student in Biomedical Sciences

Our lab implements genetic tools for studying and modulating neuronal activity. We use opto- and chemogenetic strategies together with cre-lox mice strains and/or viral constructs to target and control unique cell populations of the auditory system. We use in vitro electrophysiology (whole-cell patch clamp) that give explicit information on ion currents related to altered neuronal activity. This data is complimentary to in vivo models of intact systems. Unit recordings using acute and chronic multichannel electrodes provide new insight to neuronal activity, both sensory driven and spontaneous. Using clustering algorithms to separate units into plausible cell types we can reach new levels of understanding of regulation of neuronal activity of unique cell populations, and how alterations to neuronal activity affect circuit output. We also record auditory event-related potentials to correlate sensory gating to regulation of neuronal activity. For tinnitus research we implement auditory brainstem responses to know hearing threshold of mice, noise-overexposure to induce tinnitus without hearing loss, and we use Gap prepulse inhibition of acoustic startle tests to evaluate tinnitus perception in mice.