Richardson Leão

Our group works with circuit analysis using electrophysiology and imaging. We basically employ all the 'flavours' of patch-clamp techniques (see publications), dynamic clamp and brain modelling (from single neurons to complex networks). Regarding imaging, we work with Ca2+, Na+ and voltage imaging using dyes and we recently started to express genetically encoded voltage and Ca2+ sensors in neurons. Our group's main research topic is the study of the transfer function implemented by neurons and networks of neurons with a particular focus on the contribution of oscillations in neuronal coding in the hippocampus. Our current activities include the development of a system capable to acquire and analyse field oscillations in hippocampal slices in real time. Analysed signals are then used to modulate the firing of an artificial neuron connected to a real pyramidal cell in a closed loop configuration.


Publications


  • Malfatti T, Ciralli B, Hilscher MM, Edwards SJ, Kullander K, Leao RN, Leao KE. Using cortical neuron markers to target cells in the dorsal cochlear nucleus. eNeuro. 2021:ENEURO.0413-20.2020. Doi: 10.1523/ENEURO.0413-20.2020
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  • Winne J, Boerner BC, Malfatti T, Brisa E, Doerl J, Nogueira I, Leão KE, Leão RN. Anxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMT. Exp Neurol. 2020;326:113175. Doi: 10.1016/j.expneurol.2020.113175.
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  • Hilscher MM, Nogueira I, Mikulovic S, Kullander K, Leão RN, Leão KE. Chrna2-OLM interneurons display different membrane properties and h-current magnitude depending on dorsoventral location. Hippocampus. 2019;29(12):1224-1237.
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  • Oliveira RB, Petiz LL, Lim R, Lipski J, Gravina FS, Brichta AM, et al. Crosstalk between mitochondria, calcium channels and actin cytoskeleton modulates noradrenergic activity of locus coeruleus neurons. J. Neurochem. 2019.
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  • Moulin TC, Petiz LL, Rayêe D, Winne J, Maia RG, Lima da Cruz RV, et al. Chronic in vivo optogenetic stimulation modulates neuronal excitability, spine morphology, and Hebbian plasticity in the mouse hippocampus. Hippocampus. 2019.
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  • Mikulovic S, Restrepo CE, Siwani S, Bauer P, Pupe S, Tort ABL, et al. Ventral hippocampal OLM cells control type 2 theta oscillations and response to predator odor. Nat Commun. 2018;9(1):3638.
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  • Winne J, Franzon R, Miranda A, Malfatti T, Patriota J, Mikulovic S, Leão KE, Leão RN. Salicylate induces anxiety-like behaviour and slow theta oscillation and abolishes the relationship between running speed and fast theta oscillation frequency. Hippocampus. 2018. Doi: 10.1002/hipo.23021.
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  • Siwani S, França ASC, Mikulovic S, Reis A, Hilscher MM, Edwards SJ, et al. OLMa2 cells bidirectionally modulate learning. Neuron. 2018;99(2):404-12.e3.
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  • Lockmann AL, Laplagne D, Leão RN, Tort ABL. A respiration-coupled rhythm in the rat hippocampus independent of theta and slow oscillations. J Neurosci. 2016;36(19):5338-52.
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  • Mikulovic S, Pupe S, Peixoto HM, Nascimento GC, Kullander K, Tort ABL, et al. On the photovoltaic effect in local field potential recordings. Neurophotonics. 2016;3(1):015002.
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  • Nordenankar K, Smith-Anttila CJA, Schweizer N, Viereckel T, Birgner C, Mejia-Toiber J, Morales M, Leao RN, Walle´n-Mackenzie A (2014). Increased hippocampal excitability and impaired spatial memory function in mice lacking VGLUT2 selectively in neurons defined by tyrosine hydroxylase promoter activity. Brain Struct Funct. 11 apr. DOI 10.1007/s00429-014-0778-9
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  • Schweizera N, Pupe S, Arvidssona E, Nordenankara K, Smith-Anttila CJA, Mahmoudid S, Andrén A, Dumase S, Rajagopalan A, Lévesqued D, Leão RN, Wallén-Mackenzie A (2014). Limiting glutamate transmission in a Vglut2-expressing subpopulation of the subthalamic nucleus is sufficient to cause hyperlocomotion. PNAS. 1-6. http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1323499111/-/DCSupplemental.
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  • Scheffer-Teixeira R, Belchior H, Leão RN, Ribeiro S, Tort ABL. On high-frequency field oscillations (>100 Hz) and the spectral leakage of spiking activity. J Neurosci. 2013;33(4):1535-9.
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  • HILSCHER, M. M. ; Leão, Katarina E. ; LEAO, R. N. . Synchronization through nonreciprocal connections in a hybrid hippocampus microcircuit. Frontiers in Neural Circuits, v. 7, p. 120, 2013.
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  • ZELANO, J. ; MIKULOVIC, S. ; PATRA, K. ; KÜHNEMUND, M. ; LARHAMMAR, M. ; EMILSSON, L. ; LEAO, R. ; Kullander, K. The synaptic protein encoded by the gene Slc10A4 suppresses epileptiform activity and regulates sensitivity to cholinergic chemoconvulsants. Experimental Neurology, v. 239, p. 73-81, 2013.
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  • Leão RN, Mikulovic S, Leão KE, Munguba H, Gezelius H, Enjin A, et al. OLM interneurons differentially modulate CA3 and entorhinal inputs to hippocampal CA1 neurons. Nat Neurosci. 2012;15(11):1524-30.
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  • Leão, Richardson N. ; Colom, Luis V. ; Borgius, Lotta ; Kiehn, Ole ; Fisahn, André . Medial septal dysfunction by A -induced KCNQ channel-block in glutamatergic neurons. Neurobiology of Aging, v. 33, p. 2046-2061, 2012.
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  • Rattay, F. ; Paredes, L.P. ; Leao, R.N.. Strength duration relationship for intra- versus extracellular stimulation with microelectrodes. Neuroscience, v. 1, p. 1, 2012.
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  • Leão, Katarina E. ; Leão, Richardson N. ; Walmsley, Bruce. Modulation of dendritic synaptic processing in the lateral superior olive by hyperpolarization-activated currents. European Journal of Neuroscience (Print), v. 33, p. 1462-1470, 2011.
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  • Couchman, Kiri ; Garrett, Andrew ; Deardorff, Adam S. ; Rattay, Frank ; Resatz, Susanne ; Fyffe, Robert ; Walmsley, Bruce ; Leão, Richardson N. . Lateral superior olive function in congenital deafness. Hearing Research, v. 277, p. 163-175, 2011.
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  • Wicklund, Linn ; Leão, Richardson N. ; Strömberg, Anne-Marie ; Mousavi, Malahat ; Hovatta, Outi ; Nordberg, Agneta ; Marutle, Amelia ; Combs, Colin . ?-Amyloid 1-42 Oligomers Impair Function of Human Embryonic Stem Cell-Derived Forebrain Cholinergic Neurons. Plos One, v. 5, p. e15600, 2010.
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  • Borgius, Lotta ; Restrepo, C. Ernesto ; Leao, Richardson N. ; Saleh, Noor ; Kiehn, Ole . A transgenic mouse line for molecular genetic analysis of excitatory glutamatergic neurons. Molecular and Cellular Neurosciences (Print), v. 45, p. 245-257, 2010.
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  • Leão, Katarina E. ; Leão, Richardson N. ; Deardorff, Adam S. ; Garrett, Andrew ; Fyffe, Robert ; Walmsley, Bruce . Sound stimulation modulates high-threshold K+ currents in mouse auditory brainstem neurons. European Journal of Neuroscience (Print), v. 32, p. 1658-1667, 2010.
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  • Leão, Richardson N. ; Reis, Amilcar ; Emirandetti, Amanda ; Lewicka, Michalina ; Hermanson, Ola ; Fisahn, André ; Chin, Wei-Chun . A Voltage-Sensitive Dye-Based Assay for the Identification of Differentiated Neurons Derived from Embryonic Neural Stem Cell Cultures. Plos One, v. 5, p. e13833, 2010.
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  • LEAO, R. N. ; Tan, H. M. ; FISAHN, A. . Kv7/KCNQ Channels Control Action Potential Phasing of Pyramidal Neurons during Hippocampal Gamma Oscillations In Vitro. The Journal of Neuroscience, v. 29, p. 13353-13364, 2009.
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  • Leo, Richardson N. ; Leo, Ricardo M. ; da Costa, Luciano F. ; Rock Levinson, S. ; Walmsley, Bruce . A novel role for MNTB neuron dendrites in regulating action potential amplitude and cell excitability during repetitive firing. European Journal of Neuroscience, v. 27, p. 3095-3108, 2008.
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  • Walmsley, B. ; Berntson, Amy ; LEAO, R. N. . Activity-dependent regulation of synaptic strength and neuronal excitability in central auditory pathways. Journal of Physiology (London), v. 572, p. 313-321, 2006.
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  • Leao, K. E. ; LEAO, R. N. ; Sun, H. ; Fyffe, R. E. W. ; Walmsley, B. . Hyperpolarization-activated currents are differentially expressed in mice brainstem auditory nuclei. Journal of Physiology (London), v. 576, p. 849-864, 2006.
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  • Leão, Richardson N. ; Naves, Marcell M. ; Leão, Katarina E. ; Walmsley, Bruce. Altered sodium currents in auditory neurons of congenitally deaf mice. European Journal of Neuroscience, v. 24, p. 1137-1146, 2006.
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  • LEAO, R. N. ; Svahn, Katarina ; Berntson, Amy ; Walmsley, Bruce . Hyperpolarization-activated ( ) currents in auditory brainstem neurons of normal and congenitally deaf mice. European Journal of Neuroscience (Print), v. 22, p. 147-157, 2005.
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  • Leao, Richardson N. ; Leao, Fabricio N. ; Walmsley, Bruce . Non-random nature of spontaneous mIPSCs in mouse auditory brainstem neurons revealed by recurrence quantification analysis. Proceedings of the Royal Society of London. Biological Sciences, v. 272, p. 2551-2559, 2005.
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  • LEAO, R. N. . Topographic organization in the auditory brainstem of juvenile mice is disrupted in congenital deafness. Journal of Physiology (London), v. 571, p. 563-578, 2005.
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  • LEAO, R. N. . Continuous wavelet transform in the evaluation of stretch reflex responses from surface EMG. Journal of Neuroscience Methods, v. 133, p. 115-125, 2004.
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  • LEAO, R. N. . Reduced low-voltage activated K+ conductances and enhanced central excitability in a congenitally deaf (dn/dn) mouse. Journal of Physiology (London), v. 559, p. 25-33, 2004.
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  • LEAO, R. N. . Differences in Glycinergic mIPSCs in the Auditory Brain Stem of Normal and Congenitally Deaf Neonatal Mice. Journal of Neurophysiology, v. 91, p. 1006-1012, 2003.
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  • Lim, R. ; OLESKEVICH, S. ; FEW, A. P. ; LEAO, R. N. . Glycinergic mIPSCs in mouse and rat brainstem auditory nuclei: modulation by ruthenium red and the role of calcium stores. Journal of Physiology (London), v. 546, p. 691-699, 2002.
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  • Rattay, F ; LEAO, R. N. ; FELIX, H. . A model of the electrically excited human cochlear neuron. II. Influence of the three-dimensional cochlear structure on neural excitability. Hearing Research, v. 153, p. 64-79, 2001.
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  • Rattay, F ; LEAO, R. N. ; LUTER, P. . The Electrically Stimulated Cochlea: a Simulation Study of Potential Distribution and Excitation of the Spiral Ganglion.. Medical & Biological Engineering & Computing, v. 37, p. 802-803, 1999.
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Theses

  • VIEIRA. Hermany Munguba. Indicadores de cálcio e de voltagem codificados geneticamente na detecção de potenciais de ação e inputs sinápticos em cultura de neurônios hipocampais / Hermany Munguba Vieira. - Natal, 2013.
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