{"id":2385,"date":"2021-03-18T18:55:00","date_gmt":"2021-03-18T21:55:00","guid":{"rendered":"http:\/\/neuro.ufrn.br\/blog\/?p=2385"},"modified":"2021-03-19T18:58:09","modified_gmt":"2021-03-19T21:58:09","slug":"snapshots-in-neuroscience-fusiform-cell","status":"publish","type":"post","link":"https:\/\/neuro.ufrn.br\/blog\/index.php\/2021\/03\/18\/snapshots-in-neuroscience-fusiform-cell\/","title":{"rendered":"Snapshots in Neuroscience: Fusiform Cell"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><a rel=\"noreferrer noopener\" href=\"https:\/\/blog.eneuro.org\/2021\/03\/snapshots-mar-2021\" target=\"_blank\"><strong>SfN Staff &#8211; eNEURO blog<\/strong><\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><em>This image has been selected to showcase the art that neuroscience research can create.<\/em>As described by Dr. Thawann Malfatti and colleagues: This is a confocal image showing a neuron, likely a fusiform cell, at the dorsal cochlear nucleus (DCN) of a coronal slice from a mouse expressing CaMKII\u03b1-ChR2-eYFP along its somatic membrane and dendrites. DAPI staining of cell nuclei is shown in blue.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">This particular cryostat 30\u00b5m-thick section shows that, following injection of a rAAV5\/CamK2-hChR(H134R)-eYFP viral vector, the neuron can express and place ChR2-eYFP proteins in the membrane, allowing it to be optogenetically activated. Because only a subpopulation of neurons in the DCN express the CaMKII\u03b1 protein, this procedure allows for targeting those neurons for artificial controlling or monitoring nerve cell activity.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Using this method, we found that: neurons of different morphology are targeted, optogenetic modulation of those neurons changed the circuitry activity, and concomitant sound stimulation interferes with the optogenetically-driven changes.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Read the full article:<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/doi.org\/10.1523\/ENEURO.0413-20.2020?utm_source=EN&amp;utm_medium=Webpage&amp;utm_campaign=eneuro_blog\">Using Cortical Neuron Markers to Target Cells in the Dorsal Cochlear Nucleus<\/a><br>Thawann Malfatti, Barbara Ciralli, Markus M. Hilscher, Steven J. Edwards, Klas Kullander, Richardson N. Leao and Katarina E. Leao<\/p>\n","protected":false},"excerpt":{"rendered":"<p>SfN Staff &#8211; eNEURO blog This image has been selected to showcase the art that neuroscience research can create.As described by Dr. Thawann Malfatti and colleagues: This is a confocal image showing a neuron, likely a fusiform cell, at the dorsal cochlear nucleus (DCN) of a coronal slice from a mouse expressing CaMKII\u03b1-ChR2-eYFP along its somatic membrane and dendrites. DAPI [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":2387,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[254,2],"tags":[234,119],"class_list":["post-2385","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-ciencia","category-ice-na-midia","tag-katarina-leao","tag-kia"],"_links":{"self":[{"href":"https:\/\/neuro.ufrn.br\/blog\/index.php\/wp-json\/wp\/v2\/posts\/2385","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/neuro.ufrn.br\/blog\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/neuro.ufrn.br\/blog\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/neuro.ufrn.br\/blog\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/neuro.ufrn.br\/blog\/index.php\/wp-json\/wp\/v2\/comments?post=2385"}],"version-history":[{"count":1,"href":"https:\/\/neuro.ufrn.br\/blog\/index.php\/wp-json\/wp\/v2\/posts\/2385\/revisions"}],"predecessor-version":[{"id":2386,"href":"https:\/\/neuro.ufrn.br\/blog\/index.php\/wp-json\/wp\/v2\/posts\/2385\/revisions\/2386"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/neuro.ufrn.br\/blog\/index.php\/wp-json\/wp\/v2\/media\/2387"}],"wp:attachment":[{"href":"https:\/\/neuro.ufrn.br\/blog\/index.php\/wp-json\/wp\/v2\/media?parent=2385"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/neuro.ufrn.br\/blog\/index.php\/wp-json\/wp\/v2\/categories?post=2385"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/neuro.ufrn.br\/blog\/index.php\/wp-json\/wp\/v2\/tags?post=2385"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}